Team:Paris Liliane Bettencourt/Notebook/2011/08/10/

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Team IGEM Paris 2011

Contents

Kevin

PCR Results

Gel of PCR 2 -> 9 = LacI:GFP - 12 to 17 = tetR:YFP
Gel of PCR 1 -> 2 = tetR:YFP - 5 -> 12 = LacO Array - 15 -> 17 = tetO Array
Gel of PCR 1 -> 4 = tetO Array

We have products and lines that we expected !

Mathias & Laura

We tried to insert the Biobricks "pVeg+SpoVG T7 RFP T7 term and reporter for Bacillus" and "T7 Autoamplifier system + Reporter" into pHM3

Miniprep

-K606030

-K606036

Digestion and gel extraction

-K606030 by EcoR1 and Pst1

-T7 Autoamplifier system + Reporter (K606036) by EcoR1 and Pst1

-pHM3 by EcoR1 and Pst1

Ligation and transformation

-K606030 & pHM3

-K606036 & pHM3

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