Team:Paris Liliane Bettencourt/Notebook/2011/07/17/

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Team IGEM Paris 2011

Contents

Mathias & Laura

Miniprep

-RFP & Standard Terminator

-GFP mut3b & T7 Terminator

-pSB1C3 & RBS

Digestion and gel extraction

-RFP & Standard Terminator by Xba1 & Pst1

-GFP mut3b & T7 Terminator by Xba1 & Pst1

-pSB1C3 & RBS by Spe1 & Pst1

Ligation and transformation

-RFP Standard Terminator & RBS => K606014

-GFP mut3b T7 Terminator & RBS => K606013

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