Team:Paris Liliane Bettencourt/Notebook/2011/07/07/

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Mathias & Laura

Miniprep

-pVeg + SpoVg (K143053)

-LacI + double Terminator

Digestion and gel extraction

-pVeg + SpoVg by EcoR1 and Spe1 

-LacI + Term (new) by EcoR1 and Xba1

Transformation following the ligation pVeg + SpoVg and LacI + Term creating the new BioBrick K606010 in pSB1C3

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