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From 2011.igem.org

Dispersal of the biofilm

Aims:

To perform PCR on the rsn-2(Ranaspumin-2)and lath(Latherin) inserts.

To ligate into a suitable plasmid and transform into competent cells.

To produce successful overnight liquid cultures, then miniprep them.

To perform site directed mutagenesis on lath, thereby removing illegal restriction sites.

To ligate lath into a suitable plasmid and transform it into competent cells.

To produce successful overnight liquid cultures of lath, then miniprep them.

To devise a successful assay of the surfactant proteins.

To digest and ligate the inserts to plasmids with ribosome binding sites, the three light-dependent promoters, and double terminators present.

To characterise the latherin and ranaspumin constructs, by testing them in a biofilm setting.

To ligate the inserts into the submission vector and submit them to the registry.

Methods:

Results:

References: