Data page
How does E.D. Frosti work?
When Lactose is added to the medium, plasmid 1 will be induced to form INP via BBa_K584028.
When L-arabinose is added to the medium, plasmid 2 will be induced to form AFP via.
Data for our favorite new parts
1. BBa_K584028 (Main Page) - pLac-Lux hybrid promoter + INP , BBa_K584028 (K.U.Leuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. We added 1mM IPTG to E.D. Frosti's medium. Water freezes immediately upon addition. Addition of a control (GFP construct) E.D. Frosti did not result in freezing. Note: The promoter coupled to GFP did not show any leakiness, but when we coupled the promoter to the INP we noticed a certain leakiness. The dual inhibition system is a way to get around this leakiness.
2. BBa_K584027 (Main Page) - Constitutive Promoter + INP , BBa_K584027 (K.U.Leuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. Water freezes immediately upon addition. Addition of a control (GFP construct) E.D. Frosti did not result in freezing.
3. BBa_K584026 (Main Page) - constitutive promoter + AFP , BBa_K584026 (K.U.Leuven, iGEM 2011): We used the same conditions for the MilliQ water, but now at -10°C. Unfortunately addition of E.D. Frosti coated with AFP (or nothing: GFP control) did not result in prevention of ice formation. Due to lack of time we could not test other conditions (different temperatures, different concentrations,...)
Data for pre-existing parts
1. BBa_I13453 (Experience) - pBAD promoter, BBa_I13453 (Endy Lab, iGEM 2005): Fused a GFP reporter to this promoter (which created biobrick: BBa_K584000). The results can be found at the experience page of that promoter.
2. BBa_J23119 (Experience) - constitutive promoter, BBa_J23119 (Berkeley, iGEM 2006): Fused a GFP reporter to this promoter (which created biobrick: BBa_K584001). The results can be found at the experience page of that promoter.
We've also characterized the following parts
1. BBa_K584004 (Main Page) - HybB promoter + GFP generator, BBa_K584004 (KULeuven, iGEM 2011): We created this biobrick but due to lack of time we could not test it.
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