Team:KULeuven/Project

After stimulation, E.D.Frosti will acquire its desired biological property, i.e. the expression of Ice Nucleation Protein (INP) to induce ice formation, or the expression of a membrane-attached Antifreeze Protein (AFP) to stimulate ice melting. Upon application in the environment at low temperatures, a cell death mechanism will kill off the cells, though the desired biological activity of the proteins on the membranes will remain. This mechanism ensures that the bacteria cannot overgrow the environment.

Importantly, without any preceding stimulus, no cell death will be induced at low temperatures, so our organism can be safely stored in the fridge.