Team:UNITS Trieste/Acknowledgements

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ATTRIBUTIONS

Who helped us making synbiome possible
Research Group Who What
Vittorio Venturi, Group Leader
Bacteriology, ICGEB 		Flow cytometry
Daniel Passos, PhD Student
Bacteriology and plant bacteriology 		Flow cytometry
Mauro Giacca, Director, ICGEB Trieste Component, Group Leader
Molecular Medicine, ICGEB 		Modeling
Miguel Mano, Postdoc
Molecular Medicine, High Throughput Facility 		Data acquisition
Bruna Marini, PhD Student
Molecular Medicine 		Microscopy
Sandor Pongor, Group leader
Protein structure and bioinformatics, ICGEB 		Modeling
Adam Kerenyi, Postdoc
Biological Research Center, Szeged, Hungary 		Modeling

COOPERATIONS

"As in Synbiome also in real life collaboration is the best way to success"
Cit: "The comunicator"

Edimburg IGEM team

During the theoretical work on Synbiome we decided to use a Beta Glucosidase as carbon source for our Interkingdom Consortium. We asked to Chris French to send us the BBa_K392008 a parte made by Osaka 2010 encoding a β-glucosidase, from the bacterium Cellulomonas fimi. Firstly we tested the part and it works but during the generation of BBa_K553005 e BBa_K553006 we decided to sequence an intermediate version and the sequences underlined the presence of a frameshift at the beginning of the coding sequence, introducing a stop codon. We shared this information with Edimburg iGEM team and they elaborated a solution to this strange problem, you can find more detailed information here at the Edimburg IGEM team cooperation page. They were also so kind to provide us a protocol for an assay that can be used to test for activity of this β-glucosidase, using 4-methylumbelliferyl-β-D-glucuronide.

Wageningen IGEM team

We also collaborated with the Wageningen iGEM team analyzing with Fluorescence Microscopy a bacterial strain expressing CYP . During the summer they typed on Twitter looking for a Confocal Microscope with laser and filter for CYP. Here at ICGEB, they have it and so we decided to contact the Wageningen IGEM team in order to help them analyzing this samples.This cooperation is also reported in the Cooperation page of Wageningen’s Wiki.

JUDGING

We believe our team deserves the Gold medal because we met the following criteria:

Requirements for a Bronze Medal:
1.Register the team, have a great summer, and plan to have fun at the Regional Jamboree.
2.Successfully complete and submit this iGEM 2011 Judging form.
3.Create and share a Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.
4.Plan to present a Poster and Talk at the iGEM Jamboree.
5.Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Standard Biological Parts. Including:
5.1.Primary nucleaic acid sequence
5.2.Description of function
5.3.Authorship
5.4.Safety notes, if relevant.
5.5.Acknowedgment of sources and references
6.Submit DNA for at least one new BioBrick Part or Device to the Registry.

Additional Requirements for a Silver Medal:
1.Demonstrate that at least one new BioBrick Part or Device of your own design and construction works as expected;
2.Characterize the operation of your new part/device.
3.Enter this information and other documentation on the part's 'Main Page' section of the Registry. Part Number(s):
BBa_K553000
BBa_K553001
BBa_K553002
BBa_K553005
BBa_K553007
BBa_K553008
BBa_K553009
BBa_K553020
BBa_K553021
BBa_K553022

Additional Requirements for a Gold Medal:
1.Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system. Click here for details.
2.Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation. Click here for details.

iGEM Prizes

Best Human Practice Advance
This has been the first presence of Trieste in iGEM and this meant quite a hard venture in building up a team, finding money, finding a hosting lab, designing and developing our project.
We students have made it all, obviously with the support of the University of Trieste but mostly with our passion and determination.Here in Trieste, for its history and location, we found an ideal place in which young students like us could undertake this innovative approach to research that iGEM represents.
We invested a great amount of energy on human practices because we aimed at creating the right environment to make iGEM a core activity among the first steps of the future researchers.
We decided to talk about iGEM and about our experiences as iGEM team through different channels and in different ways referring to a wide range of audience.
You can find a detailed description of UNITS_TRIESTE team human practices here!

Team_Parts

BBa_K553020
BBa_K553008


~ iGEM Safety ~


~ Attribution and Contributions ~


Comments

IGEM, more than a competition, was a life experience to us; it has involved us as a whole, sometimes being hard, sometimes funny but above all we believe it is by far the best experience a student could embark on during his undergraduate studies.
The UNITS_TRIESTE Team can be considered a "handmade" team. No advisors working in the synthetic biology field. No company linked to any synthetic biology business. Just 6 students and their mighty wish to take their place in a worldwide acknowledged competition as iGEM is.
Synbiome is made of undergraduate ideas, and it has been realized by undergraduate hands.

As any researcher knows, three months are nothing. Only 90 days to build up a new, innovative and competitive project to face the world of synthetic biology represents quite a feat.
Still, we decided to do it as it must be done, with no shortcuts: 6 month of preliminary work turning into reality our wish to partecipate at iGEM and learning everything about it, like BioBricks, sponsorships, the registry, registration, human practice, tracks, jamboree, team and whatever else. But mostly we thought about the project, tons of rough ideas falling down like bricks (Not yet so "Bio"), looking for the best way to handle our first partecipation.
At the end of June, Synbiome was born, son of all our minds and father of all our expectations.
These lines were written only to share with you what this experience has meant for us and all the feelings it gave us. We really hope that you will enjoy Synbiome.