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Team:UPO-Sevilla/Project/Improving Flip Flop/Proteolysis/Inhibition system
From 2011.igem.org
Inhibition system
Proteolysis is a very common system to regulate gene expression in all organisms. In bacteria, proteins can be targeted for proteolysis by adding a special degradation tag in their coding sequences. That’s how the Sspb-Clpx system, which we are going to use, works.
In E.coli, ClpX recognize specific degradation tags – ssrA tags -, unfolds the attached protein and translocates this denatured polypeptide into ClpP for its degradation, which is very fast. The action of this ClpXP protease is enhanced by the SspB adaptator protein. However, it’s impossible to control proteolysis by this mechanism by manipulating SspB availability, as ClpXP protease can recognize and degrade these tagged proteins without Sspb’s aid. The degradation model is shown in Figure 1.
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