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Sensor: Week 4 June 6-11
From 2011.igem.org
Contents |
Monday
cI Repressor Test Assembly, Day 12
The minipreps prepared on 6/4 (K648000+K648001, B0030+C0051, B0032+C0051) were sent to sequencing. The sequencing results showed that the K648000+K648001 and B0030+C0051 assemblies worked. However, the B0032+C0051 assembly only produced vector background. Thus, stock should be made of the K648000+K648001 and B0030+C0051 constructs and the B0032+C0051 assembly should be redone.
Cro and OR Insert into C3 Vector, Day 1
The sensor group attempted to insert the Cro and OR parts created through PCR on 6/3 into separate C3 vectors through amplified insert assembly.
| Assembly Part | Part Involved with Insert Step | |
|---|---|---|
| Restriction Digest | Inserts using XbaI and PstI: | OR Cro |
| Vector using XbaI and PstI: | C3+violacein | |
| Ligation | OR+C3 Cro+C3 | |
| Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto chloramphenicol resistant plates. | |
Tuesday
cI Repressor Test Assembly, Day 13
The sensor group worked to make stock of their correct assemblies. The following constructs were transformed into Escherichia coli cells and allowed to grow in culture in order to make freezer stock.
K648000+K648001
B0030+C0051
B0034+C0051
Freezer stock was made from the B0031+C0051 culture as well.
