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From 2011.igem.org

Chenkai iGem Notebook

Work Stack

CHECK-add incubator

CHECK-add string name for wells and incubator slots, plates CHECK-unique identifier for each well/sample CHECK-fix duplicate restriction digests -different buffer for each protocol -using multiple tips CHECK-tip size, must specify -be generic with plate dimensions, specifying locations (grid/site), this is only with the Tecan robots CURRENT-record reaction history, also protocol history -move transversing the tree out of assemblymanager, we want to a separate **program to annotated the tree and produce the protocol graph -tell user when new plate gets allocated CHECK-if two wells have partial amounts, combine them CURRENT-debugging purposes, log everything that happens -why does resourcemanager give the printout out of order? investigate -fix naming, 3 parts (UUIDS,User defined names,auto-generated linearized tree name) CHECK-swap column and row -save resourcemanager state -Talk to Jeno about Puppetshow GUI

Work Diary

• Wednesday 6/8

Just flew back from China, got to PHO209 at 4pm and caught up with other team members on what they are up to. Had a discussion with Viktor about the road ahead, what problems are on our to do list.

• Thursday, 6/9

Found a fix to the sample name duplication issue, instead of using sample names to identify sample, we are now adding a new UUID field to each liquid, this 8 digit randomly generated code will make sure two no samples of the same content will be confused with each other.

• Friday 6/10

Implemented tips of different sizes, 50ul, 100ul, 200ul Removed duplicate entries from Puppeteer output Implemented incubator functionality

• Monday 6/13

Planned and implemented history recording, reaction history and incubation history Got the robot moving and tested in the wetlab

• Tuesday 6/14

Swapped rows with columns to optimize robot pippetting Added feature to ResourceManager to combine liquids in multiple wells to minimize waste

• Wednesday 6/15