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Team:Potsdam Bioware/BioBricks
From 2011.igem.org
BioBricks
BioBrick mdnDE
Part name: BBa_K627000
Part type: Composite
Short description: mdnDE - N-acetyltransferase and ABC transporter
Full description:
The BioBrick mdnDE is a part of the whole microviridin gene (mdn) cluster, which encodes the protease
inhibitor microviridin L. Microviridins are tricyclic depsipeptides, which are ribosomally synthesized
by Microcysistis (Ziemert et al. 2010). They have a promising potential for therapy as they can block
disease-relevant proteases (Ziemert et al. 2008).
Microviridins are synthesized from a ribosomal precursor peptide (MdnA). Additionally, the microviridin L
biosynthesis gene cluster consists of genes encoding an ATP-grasp-type ligase (mdnB and mdnC) and genes,
which encode an ABC transporter (mdnE) and a N-acetyltransferase of the GNAT family (mdnD) (Ziemert et al.
2008).
In the following BioBrick mdnDE the genes mdnD (N-acetlytransferase of the GNAT family) and mdnE (ABC
transporter) is encoded (Ziemert et al. 2008).
Because this BioBrick is an expression part, the adenin of mdnE gene's start codon is part of the XbaI
recognition site.
Source of the part:
The microviridin gene (mdn) cluster was isolated from Microcystis aeruginosa strain NIES-843.
References:
Ziemert, N., Ishida, K., Liaimer, A., Hertweck, C. & Dittmann, E. (2008). Ribosomal synthesis of tricyclic depsipeptides in bloom-forming cyanobacteria. Angewandte Chemie (International ed. in English) 47, 7756-9
Ziemert, N., Ishida, K., Weiz, A., Hertweck, C. & Dittmann, E. (2010). Exploiting the natural diversity of microviridin gene clusters for discovery of novel tricyclic depsipeptides. Applied and environmental microbiology 76, 3568-74
Genebank file
