Team:BU Wellesley Software/Notebook/AlbertoNotebook

From 2011.igem.org

2011 IGEM WetLab Notebook

Weekly Log

6/6/11-10/6/11

A. BFP2

Plasmid prep were created from the BFP2 culture plate and incubated for approximately 16 hours for plasmid amplification. The resulting plasmids were isolated by miniprep and quantified with nanodrop. The DNA concentration in the two samples are 16.5 ng/uL and 14.9 ng/uL, respectively. While such values are below the normally acceptable range of 20 and above, we still decided to run electrophoresis on them. No band is detected in the gel.

B. Pbad

Using the IGEM plate from spring 2010, Pbad promoter biobrick was obtained and transformed into ecoli bacteria. The bacteria were then plated onto agar-based LB+Ampicillin growth medium. Lots of cultures were found on the next day after the overnight transformation. Plasmid prep was