Week 1

First Primers arrived. Doing lots of PCRs.

Week 2

Still doing PCRs and start restriction digests.

Week 3

First Biobrick is ready!

Week 4

Week 5

Week 6

Week 7

Week 8

Protocols

Primer

pnikA-E,N,X-fwd GCAGAATTCGCGGCCGCTTCTAGAGTTAAGCCTTGCGATCTGCACC
pnikA-S-rev CCGCTACTAGTAGACGATAAAAGACGCACAAGCC
prcnA-E,N,X-fwd GCAGAATTCGCGGCCGCTTCTAGAGacggattgtatgagacatggca
prcnA-S-rev CCGCTACTAGTAcgcaccaagtaagatggcg
luxCDrev CTGCAGCGGCCGCTACTAGTATTAAGACAGAGAAATTGCTTGAT
PbrR-M,R-fwd GCAGAATTCGCGGCCGCTTCTAGAGAAGAAGGAGATATACCATGAATATCCAGATCGGCGAG
PbrR-S,A-rev AGCCTGCAGCGGCCGCTACTAGTAttaCTAGTCGCTTGGATGGGCG
pbrRT-S,A-rev agtcactagtattaaccggttaTTACACCTGGGTAGATGGCC
pbRMut-fwd tcgtgcgggattctccagggactgtcggactgc
pbrMut-rev tccgacagtccctggagaatcccgcacgattgggc
ppbrA-E,N,X-fwd AGCCTGCAGCGGCCGCTACTAGTAGGTTGCGCGTCGCAACGGAAGC
ppbrA-S-rev GCAGAATTCGCGGCCGCTTCTAGAGCATGCGGTGCGCTTGGCAAGC
luxCDfor GAATTCCGCGGCCGCTTCTAGATGGAAAATGAATCAAAATA
luxBfor ATGAAATTTGGATTGTATGAAATTTGGATTGT
luxBrev CTGCAGCGGCCGCTACTAGTATTAGGTATATTCC
luxEfor GAATTCCGCGGCCGcttctagaATGTGACTGGGGTGAGTGA
luxErev CTGCAGCGGCCGCTACTAGTACTATCAAACGCTTCGGTTAA
iscSfor AgtcgccggcAAGAAGGAGATATACCATGTACGGAGTTTATAGAGC
icsSrev agtcactagtattaaccggtctattaatgatgagcccattcg
pabAfor AgtcgccggcAAGAAGGAGATATACCATGAAATTGCTATTAATTGATAATTATG
pabArev agtcactagtattaaccggtTTATTACACCACTTTCAAAAAATTATTTAAC
aurFfor AgtcgccggcAAGAAGGAGATATACCATGCCACGACACCGCGGGC
aurFrev agtcactagtattaaccggttaTCAACGCGGCGTGTGGGGCG
ChrBAfor AgtcgccggcAAGAAGGAGATATACCATGAACGCTCTCCCATCCTC
ChrBArev agtcactagtattaaccggttaTCAGTGATGCAACAACGGATAGG
melAfor gatcTCTAGAtgGCCGGC GCGTGGCTGGTCGGCAAGCCG
melArev gatcACCGGT GGCGGACACTATGGCTATTTCTAGC