Week 1

First Primers arrived. Doing lots of PCRs.

Week 2

Still doing PCRs and start restriction digests.

Week 3

First Biobrick is ready!

Week 4

Week 5

Week 6

Week 7

Week 8

Protocols

Primer

pnikA-E,N,X-fwd: GCAGAATTCGCGGCCGCTTCTAGAGTTAAGCCTTGCGATCTGCACC
pnikA-S-rev: CCGCTACTAGTAGACGATAAAAGACGCACAAGCC
prcnA-E,N,X-fwd: GCAGAATTCGCGGCCGCTTCTAGAGacggattgtatgagacatggca
prcnA-S-rev: CCGCTACTAGTAcgcaccaagtaagatggcg
luxCDrev: CTGCAGCGGCCGCTACTAGTATTAAGACAGAGAAATTGCTTGAT
PbrR-M,R-fwd: GCAGAATTCGCGGCCGCTTCTAGAGAAGAAGGAGATATACCATGAATATCCAGATCGGCGAG
PbrR-S,A-rev: AGCCTGCAGCGGCCGCTACTAGTAttaCTAGTCGCTTGGATGGGCG
pbrRT-S,A-rev: agtcactagtattaaccggttaTTACACCTGGGTAGATGGCC
pbRMut-fwd: tcgtgcgggattctccagggactgtcggactgc
pbrMut-rev: tccgacagtccctggagaatcccgcacgattgggc
ppbrA-E,N,X-fwd: AGCCTGCAGCGGCCGCTACTAGTAGGTTGCGCGTCGCAACGGAAGC
ppbrA-S-rev: GCAGAATTCGCGGCCGCTTCTAGAGCATGCGGTGCGCTTGGCAAGC
luxCDfor: GAATTCCGCGGCCGCTTCTAGATGGAAAATGAATCAAAATA
luxBfor: ATGAAATTTGGATTGTATGAAATTTGGATTGT
luxBrev: CTGCAGCGGCCGCTACTAGTATTAGGTATATTCC
luxEfor: GAATTCCGCGGCCGcttctagaATGTGACTGGGGTGAGTGA
luxErev: CTGCAGCGGCCGCTACTAGTACTATCAAACGCTTCGGTTAA
iscSfor: AgtcgccggcAAGAAGGAGATATACCATGTACGGAGTTTATAGAGC
icsSrev: agtcactagtattaaccggtctattaatgatgagcccattcg
pabAfor: AgtcgccggcAAGAAGGAGATATACCATGAAATTGCTATTAATTGATAATTATG
pabArev: agtcactagtattaaccggtTTATTACACCACTTTCAAAAAATTATTTAAC
aurFfor: AgtcgccggcAAGAAGGAGATATACCATGCCACGACACCGCGGGC
aurFrev: agtcactagtattaaccggttaTCAACGCGGCGTGTGGGGCG
ChrBAfor: AgtcgccggcAAGAAGGAGATATACCATGAACGCTCTCCCATCCTC
ChrBArev: agtcactagtattaaccggttaTCAGTGATGCAACAACGGATAGG
melAfor: gatcTCTAGAtgGCCGGC GCGTGGCTGGTCGGCAAGCCG
melArev: gatcACCGGT GGCGGACACTATGGCTATTTCTAGC