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From 2011.igem.org

• Home
• About us
  • The Team
  • Supervisors
  • Gallery
• The Project
  • Abstract
  • Introduction
  • The Plug 'n' Play
    • Assembly system
    • Customization
  • Other assembly systems
  • Achievements
  • Attributions
• Results
  • Background
  • Characterization
  • Proof of concept
    • Mammalian Cells
    • Fungi
  • Data page
  • Submitted Biobricks
  • Collaboration
• Safety
  • Safety
  • Safety rules
• Notebook
  • Lab notes
  • Protocols
• Our Partners
  • Sponsors
  • Advisors
  • Acknowledgements

Our mission insert text here! We have achieved to create a novel assembly standard system for BioBrick called Plug'n Play with DNA. We submitted 49 new parts and 21 plasmids to the Registry of Standard Biological Parts. We demonstrated the function of our system in the mammalian cell line U2-OS by fluorescence and confocal microscopy. We demonstrated the function of our system in filamentous fungi Aspergilli by fluorescence and microscopy. We demonstrated the function of our system in E. coli by helping the Copenhagen iGEM team. We achieved to characterized two new fungal promoter by use of ß-galactosidase assays and Bradford assays. Why Plug 'n' Play with DNA? We have developed a standardized assembly system, called “Plug’n’Play with DNA”, where any biological parts can be gathered without use of restriction enzymes and ligases. The method applies long complementary overhangs on the PCR product as well as the destination vector. These overhangs are custom made and can anneal to each other to form a stable hybridization product. This means that the parts in the form of pre-produced PCR-products are directly mixed with a vector, which makes assembly of an expression vector possible within a few hours. All the parts in the form of pre-produced PCR-products will be distributed in microtiter plates directly ready for cloning. The simple and easy use of the system was demonstrated by developing a reporter targeting system for the mammalian cell line, U2OS, where GFP was targeted to the peroxisomes. In addition, application of our system was demonstrated in the fungus Aspergillus nidulans.

iGEM 2011 Wiki Main Page

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