Team:DTU-Denmark-2/Project/achievements

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Achievements

Even though we first started the iGEM project in the end of June, we have managed to design a fully standardized cloning system called Plug'n Play with DNA. We think that iGEM should be about fast and easy assembling of BioBricks, which led us to use features from the USER fusion standard assembly to model our Plug'Play with DNA assembly system. Our system consist of:

  • Ready PCR products - All ready to use!
  • Back-up plasmide - To ensure mutation free amplification.
  • Guide on customization - All procedures only require 1 round of PCR and assembly To proof our concept, we have characterized the two fungal promoters, PalcA and DMKP-P6, and ___ mammalian promoters, ________ __________ . The characterization of the fungal promoters was performed by evaluation of the qualitative X-gal analysis, where the expression of ß-galactosidase results in blue colonies. Furthermore, quantitative ß-galactosidase and Bradford assay was performed to measure the protein production.

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