"
Team:HokkaidoU Japan/Project
From 2011.igem.org
Contents |
Intro
Bacteria living around us evolved ways to effect their surrounding environment. This is done by using secretion systems. There are six known types of secretion systems: one through six. These are called Type One Secretion System (T1SS), T2SS, T3SS and so on. Some system only secrete designated proteins outside others can translocate proteins directly to other cells. Secretion systems able to inject proteins into other cell caught many researchers imagination.
Ours was no exception. We chose to use T3SS for our experiments. During iGEM 2010 we found that E.Coli with a part of Salmonella genome library expresses T3SS. This presented opportunity to work with the amazing machinery without involving pathogenic bacteria.
We repeated previous competitions experiment, one more time showing that GFP can be really injected into a target cell with it. And then continued to characterise it. An further develop data sheet of T3SS possibilities.
Type Three Secretion System Data Sheet
Still to come.
Plasmid Backbone for protein injection
We developed plasmid backbone which can attach tags needed for secretion and various other functions to a chosen protein biobrick. This can be used for big scale screening of various protein domains for their inject-ability.
More info still to come.
Structure of Injectable Proteins
Here we will discus the structure of proteins which are injected. Still to come.
