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RecA: Week 3, May 30-June 3
From 2011.igem.org
Contents |
Monday
RecAI Extraction, Day 1
The RecA group extracted the RecAI gene from Escherichia coli using the genomic DNA extraction kit produced by Omega Bio-Tek. The RecA group created a culture of the RecAI gene and left this culture to grow overnight.
Tuesday
RecAI Extraction, Day 2
The RecA group performed miniprep on the RecAI culture from 5/30/11. The RecA group placed the RecAI gene into the C3 vector through amplified insert assembly in order to perform mutagenesis on the RecAI gene. The RecAI team also developed a plan for RecA mutagenesis. Figure 1 shows the overview of this plan.
