Team:Washington/Alkanes/Future

From 2011.igem.org

Our current in vivo system only efficiently makes C15 alkanes. To be efficient enough for factory production, there are two broad goals to be done: 1. increase production efficiency 2. Diversity the range of alkanes for the system. 3. Increase scale of system for industrial processes. We have already begun efforts to expand the efficiency and scope of alkane production.

Decarbonylase Redesign

One way to diversify the kind of alkanes produced is to alter the substrate specificity of the proteins involved. We decided to alter the aldehyde decarbonylase to produce shorter-chain fatty aldehydes.

Alternate Aldehyde Production

Another way to diversify our system is to use alternative proteins. Our current system uses acyl-ACP reductase, and we've identified an hypothetical alternative system that produces aldehydes: LuxCDE, made from parts from the 2010

Branched Alkanes Production

System Optimization

Enzyme Localization

Alternative Chasis

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