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Sensor: Week 3 May 30-June 4
From 2011.igem.org
Contents |
Monday
cI Repressor Test Assembly, Day 6
The sensor group ran PCR, transformed, and cultured ribosome binding sites from the registry as well as the cI repressor. The sensor group also prepared for miniprep and sequencing for tomorrow.
| Assembly step | Parts Ivolved with Assembly Step |
|---|---|
| PCR | C0051 from kit |
| Transform/Culture | B0030 B0032 C0051 from kit |
| Colony Growth (for sequencing) | B0031+C0051 B0033+C0051 |
Tuesday
cI Repressor Test Assembly, Day 7
The sensor group performed miniprep from the cultures created 5/30/2011. Two RBS and cI repressor constructs were sent to sequencing while assembly was repeated for the other two RBS and the cI repressor since this assembly from last week failed.
| Assembly Step | Parts Involved with Assembly Step | |
|---|---|---|
| Miniprep | For Sequencing: | B0031+C0051 B0033+C0051 |
| For Assembly: | C0051 B0030 B0032 | |
| Restriction Digest | Insert using XbaI and PstI: | C0051 |
| Vector using SpeI and PstI | B0030 B0032 | |
| Ligation | B0030+C0051 B0032+C0051 | |
| Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto Amp resistant plates. | |
Wednesday
cI Repressor Test Assembly, Day 8
The sensor group placed constitutive promoters in front of the RBS+cI repressor construct and attatched another RBS to the cI repressor.
| Assembly Step | Parts Involved in Assembly Step | |
|---|---|---|
| Restrction Digest | Inserts using XbaI and PstI: | J23113 J23115 C0051 |
| Vectors using SpeI and PstI: | B0033 B0031+C0051 | |
| Ligation | J23113 + B0031+C0051 J23105 + B0031+C0051 B0033 + C0051 | |
| Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto Amp resistant plates. | |
