Team:Lethbridge/Notebook/Lab Work/Group3

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=June=
==June 21st==

Restriction, Ligation, and Transformation
Lumazine synthase - double terminator and pBAD - RBS into pSB1C3

  1. Restriction
    • Restricted lumazine synthase - dt out of pSB1K3 and pBAD - RBS out of pSB1T3 with EcoR1 and Pst1
    • Restricted destination plasmid pSB1C3 with EcoR1 and Pst1
    1. Ligation
    • Ligated lumazine synthase-dt into destination plasmid pSB1C3
    • Ligated pBAD-RBS into destination plasmid pSB1C3
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