Team:Lyon-INSA-ENS/Realisation/Protocols/Storage
From 2011.igem.org
Storage of strains
This protocol aims at storing strains at -20°C for long-time conservation.
Procedure
1. In a 1.5mL eppendorf tube, add 750µL of overnight LB culture and 250µL glycerol 80%.
2. Store in a -20°C freezer.