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Team:WarrenCIndpls IN-HS/Notebook
From 2011.igem.org
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Contents |
Notebook
May 4th: Bacterial and Yeast Transformation
May 11th: Gibson Assembly
May 19th: Research on Parts
Kozak Sequence- directs translation of mRNA for more efficiency and accuracy; the amount of protein synthesized from mRNA is dependent on the strength of the Kozak sequence
Promoter Region- site for RNA polymerase to attach to and begin transcription; yeast has many promoters within its genome that can be used to express metal detectors
Terminator Sequence- signals the end of transcriptoin to RNA polymerase
Vector- Plasmid
ORI (Origin of Replication)
Selection Markers
-Ura3 is a selection marker for yeast
-Ampicillin Resistance is a selection marker for bacteria based antibiotic resistance
May 26th: Primer Design
1st Forward Primer contains an overhang to attach the biobrick to the plasmid, two restriction enzyme sites (required to form a biobrick), and a primer for the constructin of a new strand
2nd Forward Primer contains a primer for contructing a new strand to connect the translational unit to the biobrick
Reverse Primer contains an overhang to attach the biobrick to the multi-cloning site (on the plasmid), the other two restriction enzymes sites, and a primer for contructiong a new strand
