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From 2011.igem.org

Contents 1 Danyel 1.1 S38 in S24 1.2 Sequencing results 1.3 Ligation & Transformation 2 Cyrille and Antoine

Danyel

S38 in S24

Transformation failed. Positive control and negative control were good but no colonies for neither 20µL nor 200µL.

Sequencing results

• S98 : clone 1 and 6
• S99 : clone 2
• S92 : the results are correct

Ligation & Transformation

• S79 in S27
• S105 in S27 x2 (one of the MPs didn't show satisfactory sequencing results)
• S27 (-)ctrl for the two tubes that I used. (one concentrated at 9.8, the other at 5.4)

Cyrille and Antoine

Preparation of the last step of the ligation.

12 miniprep of pHM3 were done and concentration measured. 2 times 500 ng of each plasmid was digested in EP and runned on a gel. The bands where unclear. Only one miniprep seems to hold the correct insert altough there is a blur band for each of them at thee good size.this band was extracted and DNA was purified.

Treatment of the sequences - kinA project abandonned due to a wrongregsitry part

Digestion of PVeg ComS GA/PCR in EX, all the trnas (the digestion failed once), RBS frp ter, pT7 GFP in EX and SP (failed), S24 CI