"
Team:Cornell/Week 15
From 2011.igem.org
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
September 11th - September 17th
Sunday, September 11
~God bless America -- never forget~
Monday, September 12
Afternoon lab work done by: Nancy Li, Charlie Chung
- Prepared ten 3mL LB cultures (with 3µL carbenicillin) of colonies picked from plates dated Sept 3
- - RFP + Avi-Tagged pZE12 in DH5α
- - vioA + Avi-Tagged pZE12 in DH5α
- - vioB + Avi-Tagged pZE12 in DH5α
- - vioE + Avi-Tagged pZE12 in DH5α
- - RFP + Avi-Tagged pZE12 in MC4100
- - vioA + Avi-Tagged pZE12 in MC4100
- - vioB + Avi-Tagged pZE12 in MC4100
- - vioE + Avi-Tagged pZE12 in MC4100
- - Control tube is just a colony with pZE12 (no insert gene)
- - LB with carbenicillin (no inoculation with bacteria)
- Incubating at 37°C in the shaker at Weill Hall
- Cultures are to be lysed for release of natively biotinylated RFP and vio enzymes in characterizing streptavidin-coated microfluidics chips
- Did not extensively search for L-tryptophan (starting substrate in the synthesis of prodeoxyviolacein) in the reagents shelf of our Weill Lab space, but does not appear to be in plain view (may be tucked away in an obscure corner?)
