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From 2011.igem.org

Contents 1 Monday 2 Tuesday 3 Wednesday 4 Thursday 5 Friday

Monday

Assembly Step	Parts Involved in Assembly Step
PCR	J23113 J23105 J23118 J23100

Tuesday

Assembly Step	Parts Involved in Assembly Step
PCR Purification	J23113 J23105 J23118 J23100
Miniprep	B0051
Restriction Digest	Inserts using EcoRI and SpeI:	J23113 J23105 J23118 J23100 B0034+Mcherry
	Vectors using EcoRI and XbaI:	B0034+C0051 B0015
Ligation	J23113 and B0034+C0051 J23105 and B0034+C0051 J23118 and B0034+C0051 J23100 and B0034+C0051 B0034+Mcherry and B0015
Transformation/Plating	The above ligations were transformed into Escherichia coli cells and plated onto Amp resistant plates.
PCR	B0030 B0031 B0032 B0033

Wednesday

Assembly Step	Parts Involved with Assembly Step
Colony PCR	B0034+Mcherry+B0015 (Kan resistance) B0034+Mcherry+B0015 (Amp resistance)
PCR Purification	B0030 B0031 B0032 B0033
Restriction Digest	Inserts using EcoRI and SpeI:	B0030 B0031 B0032 B0033
	Vector using EcoRI and XbaI:	C0051
Ligation	B0030+C0051 B0031+C0051 B0032+C0051 B0033+C0051
Transformation/Plating	The above ligations were transformed into Escherichia coli cells and plated onto Amp resistant plates.

Thursday

Assembly Step	Part Involved with Assembly Step
Miniprep for Sequencing	B0034+Mcherry+B0015 J23113+B0034+C0051 J23105+B0034+C0051
PCR	B0034+Mcherry+B0015
Restriction Digest	Insert using EcoRI and SpeI:	B0034+Mcherry+B0015
	Vectors using EcoRI and XbaI:	J23113+B0034+C0051 J23105+B0034+C0051
Ligation	B0034+Mcherry+B0015 and J23113+B0034+C0051 B0034+Mcherry+B0015 and J23105+B0034+C0051
Transformation/Plating	The above ligations were transformed into Escherichia coli cells and plated onto Amp resistant plates.

Note: The minipreps in green font indicate the sequencing proved the assembly worked.

Friday

The following assemblies have been confirmed and named for the registry.

Assembly	Registry Name
B0034+mCherry+B0015 J23113+B0034+C0051 J23105+B0034+C0051	K648000 K648001 K648002

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