Team:NYC Wetware/Notebook/Week9

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NYC-iGEM wetware

7/18/2011
Ran gel on the final 10 E. coli genes that still needed extraction. Separation and use of transilluminator successful. Extracted bands of interest. Ran colony PCR to test out our new D. rad primers.

7/19/2011
Yesterday's D. rad colony PCR FAILED. Re-runs with extracted DNA from early-summer trials and with higher concentration of colonies. Re-runs FAIL too.

Extracted DNA from yesterdays gels and ligated them into plasmid for transformation tomorrow.