Sensor: Week 2 May 23-27
From 2011.igem.org
Monday
Assembly Step | Parts Involved in Assembly Step |
---|---|
PCR | J23113 J23105 J23118 J23100 |
Tuesday
Assembly Step | Parts Involved in Assembly Step | |
PCR Purification | J23113 J23105 J23118 J23100 | |
Miniprep | B0051 | |
Restriction Digest | Inserts using EcoRI and SpeI: | J23113 J23105 J23118 J23100 B0034+Mcherry |
Vectors using EcoRI and XbaI: | B0034+C0051 B0015 | |
Ligation | J23113 and B0034+C0051 J23105 and B0034+C0051 J23118 and B0034+C0051 J23100 and B0034+C0051 B0034+Mcherry and B0015 | |
Transformation/Plating | The above ligation was transformed into Escherichia coli cells and plated onto Amp resistant plates. |