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From 2011.igem.org

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Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions

Contents 1 Notebook 2 May 4th: Bacterial and Yeast Transformation 3 May 11th: Gibson Assembly 4 May 19th: Research on Parts 5 May 26th: Primer Design

Notebook

May 4th: Bacterial and Yeast Transformation

May 11th: Gibson Assembly

Gibson Assembly pdf

May 19th: Research on Parts

Kozak Sequence- directs translation of mRNA for more efficiency and accuracy

Promoter Region- site for RNA polymerase to attach to and begin transcription; yeast has many promoters within its genome that can be used to express metal detectors

Terminator Sequence- signals the end of transcriptoin to RNA polymerase

Vector- Plasmid

    ORI (Origin of Replication)
    Selection Markers
         -Ura3 is a selection marker for yeast
         -Ampicillin is a selection marker for bacteria

May 26th: Primer Design