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From 2011.igem.org

• Home
• About us
  • The Team
  • Supervisors
  • Gallery
• The Project
  • Abstract
  • Introduction
  • The Plug 'n' Play
    • Assembly system
    • Customization
  • Other assembly systems
  • Achievements
  • Attributions
• Results
  • Background
  • Characterization
  • Proof of concept
    • Mammalian Cells
    • Fungi
  • Data page
  • Submitted Biobricks
  • Collaboration
• Safety
  • Safety
  • Safety rules
• Notebook
  • Lab notes
  • Protocols
• Our Partners
  • Sponsors
  • Advisors
  • Acknowledgements

We know how important flexibility within molecular biology is, however setting up a standard often entails rigidity.
We therefore here provide a guide on how to customize the Plug’n’Play assembly standard.


When do you need to customize the system?

• When a desired biological part is not yet a part of the Plug’n’Play with DNA kit
• When scar-free assembly is required (e.g. protein fusion)
• When you want to introduce mutations

	Creating new parts Scar-free assembly Site-directed mutagenesis	Creating new parts Seamless assembly Site-directed mutagenesis Retrieved from "http://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly/customization" Recent changes What links here Related changes Special pages My preferences Permanent link Privacy policy Disclaimers