Team:Osaka/week6

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Contents

September 4(Sun)

  1. Restriction digests:3-10C,1-2M
  2. Gel extraction of digests
  3. Ligation
    • 03:1-9N+3-6H again
  4. Transformation of ligation products
  5. Photospectrometry measuament

September 5(Mon)

  1. Absorbance measurement
Table 7
NameIPTG(0.1M)incubationOD600A474
1 --0.85830.1567
2400μl2h1.15230.1447
3400μl1h0.55830.1126
440μl2h1.02970.1878
540μl1h0.65760.1736
  1. To liquid culture:03,17,3-10C


September 6(Tue)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • all parts ×
  4. PCR to amplify PprI,PprA,PprM,RecA (DNA repair protein) from D.radiodurance
  5. Gel Electrophoresis of PCR products
    • PprM ok
    • PprI,PprA,RecA ×
  6. PCR
  7. Gel Electrophoresis of PCR products
    • ×


September 7(Wed)

  1. PCR
  2. Gel electrophoresis of PCR products
    • PprM ok
    • PprI,PprA,RecA ×
  3. Gel electrophoresis of PprM
  4. Gel extraction
  5. PCR
  6. Gel electrophoresis of PCR products
    • PprI,PprA,RecA ×
  7. Miniprep of yesterday's culture
  8. Restriction digests of miniprepped parts
  9. Gel electrophoresis of digests
    • 03 ok
  10. To liquid culture:03,04 (preincubation)

September 8(Thu)

September 9(Fri)

September 10(Sat)

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