Team:Osaka/week6
From 2011.igem.org
Contents |
September 4(Sun)
- Restriction digests:3-10C,1-2M
- Gel extraction of digests
- Ligation
- 03:1-9N+3-6H again
- Transformation of ligation products
- Photospectrometry measuament
September 5(Mon)
- Absorbance measurement
Name | IPTG(0.1M) | incubation | OD600 | A474 |
---|---|---|---|---|
1 | - | - | 0.8583 | 0.1567 |
2 | 400μl | 2h | 1.1523 | 0.1447 |
3 | 400μl | 1h | 0.5583 | 0.1126 |
4 | 40μl | 2h | 1.0297 | 0.1878 |
5 | 40μl | 1h | 0.6576 | 0.1736 |