Team:Osaka/week6
From 2011.igem.org
Contents |
September 4(Sun)
- Restriction digests:3-10C,1-2M
- Gel extraction of digests
- Ligation
- 03:1-9N+3-6H again
- Transformation of ligation products
- Photospectrometry measuament
September 5(Mon)
|+Table 1 !name!!IPTG(0.1M)!!OD600!!incubate!!A<math>474</math> |- |1||400μl||0.8583||-||0.1567 |- |1-2M||<bbpart>BBa_K118022</bbpart>||A||RBS |- |1-14K||<bbpart>BBa_B0040</bbpart>||A||green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP |- |1-12M||<bbpart>BBa_E0240</bbpart>||A||GFP generator |- |1-1D||<bbpart>BBa_R0010</bbpart>||A||promoter(lacI regulatd) |- |}