18 (May 18, 2011 GDS)

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Parth--made fresh TENS

4.5 TE
250λ 10% SDS
250λ 2M NaOH
Minipreps (2 each) for:
pNICE SA-15λ TE
J024430 GE-15λ TE
Trans002-5, AB-Resp 30&lambda
Trans003-W1 SD, SA-
Spin 1.5 mL (2 tubes) 30"
Pour off sol'n + resuspend
Add: 300λ TENS mix
150λ NaOAc mix
Spin 2.5'
Sup to new tube
Add 1 mL ETOH - mix well
Spin 3' ________
Wash 70% ETOH
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