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From 2011.igem.org

Contents 1 Kevin 1.1 Biobrick the YFP:tetR fusion protein 1.1.1 PCR 1.1.2 Following steps 1.1.3 To be continued

Kevin

Biobrick the YFP:tetR fusion protein

Let's start again with good primers to biobrick the protein.

PCR

3 Temperatures of primer annealing: 85 - 58 - 48 °C
Primers : pFX234 FW - pFX234 Reverse CP

Following steps

PCR Purification, digestion in ES, PCR Purification again, Ligation

To be continued

Transformation, sequencing and glycerol.