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From 2011.igem.org

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Safety proposal

1. Would any of your project ideas raise safety issues in terms of:

• researcher safety,
• public safety, or
• environmental safety?

   Our study will not contain any manipulation associated with pathogenic bacteria, live salmonella bacteria. Although we are planning to use a part of Salmonella's genome which was obtained from Salmonella Genetic Stock Centre (SGSC), our instructor obtained appropriate permission from the safety officer of genetic recombination in our university. These E. coli were acknowledged as non-pathogenic and permitted to be used under P1 safety level.

   Our lab is equipped appropriately for the manipulation and genetic recombination of bacterial cells. Team members are instructed according to the safety training manual.

2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,

• did you document these issues in the Registry?
• how did you manage to handle the safety issue?
• How could other teams learn from your experience?

   Currently there are no BioBricks which raise any safety issues.

3. Is there a local biosafety group, committee, or review board at your institution?

• If yes, what does your local biosafety group think about your project?
• If no, which specific biosafety rules or guidelines do you have to consider in your country?

   We have “the Safety Office of Genetic Recombination in Hokkaido University”.

4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

   Currently we don't have any suggestions.

<-- Further Questions

1. Would the materials used in your project and/or your final product pose:

• Risks to the safety and health of team members or others in the lab?
• Risks to the safety and health of the general public if released by design or accident?
• Risks to environmental quality if released by design or accident?
• Risks to security through malicious misuse by individuals, groups or states?

   We are using following parts of salmonella genome library. BAC clones of S. Typhimurium, B_STM02P01　SGSC4014　837453-941863 B_STM07H21 SGSC4024 1464540-1562427

These were obtained from SGSC. SGSC4024 contains Salmonella Pathogenecity Island Two (SPI2) coding sequence which codes for Type Three Secretion System (T3SS) used after Salmonella invades cell. For salmonella to be pathogenic T3SS coded on Salmonella Pathogenecity Island One (SPI1) is required. So there is no threat of the E.coli with this BAC vector to be pathogenic. SGSC4014 doesn't code neither SPI1 nor SPI2 so it also doesn't rise safety concerns.

2. If your response to any of the questions above is yes:

• Explain how you addressed these issues in project design and while conducting laboratory work.
• Describe and document safety, security, health and/or environmental issues as you submit your parts to the Registry.

3. Under what biosafety provisions will / do you operate?

• Does your institution have its own biosafety rules and if so what are they? Provide a link to them online if possible.
• Does your institution have an Institutional Biosafety Committee or equivalent group? If yes, have you discussed your project with them? Describe any concerns or changes that were made based on this review.
• Will / did you receive any biosafety and/or lab training before beginning your project? If so, describe this training.
• Does your country have national biosafety regulations or guidelines? If so, provide a link to them online if possible.

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