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User contributions
From 2011.igem.org
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- 02:03, 19 September 2011 (diff | hist) Team:Washington/Magnetosomes/Results
- 01:04, 19 September 2011 (diff | hist) Team:Washington/Magnetosomes/Results (→What' in the toolkit and How We Assembled It)
- 23:11, 18 September 2011 (diff | hist) Team:Washington/Magnetosomes/Results
- 20:09, 16 September 2011 (diff | hist) Team:Washington/Magnetosomes/Results
- 01:06, 16 September 2011 (diff | hist) Template:Team:Washington/Templates/Top
- 00:49, 16 September 2011 (diff | hist) Team:Washington/Team/Members (→Gibson Vectors)
- 00:22, 16 September 2011 (diff | hist) Team:Washington/Magnetosomes/Results
- 22:39, 15 September 2011 (diff | hist) Team:Washington/Protocols (→Make It: iGem Toolkits)
- 22:38, 15 September 2011 (diff | hist) Team:Washington/Protocols/Induc studies. (top)
- 22:36, 15 September 2011 (diff | hist) Team:Washington/Protocols (→Make It: Magnetosome Protocols)
- 22:31, 15 September 2011 (diff | hist) N Team:Washington/Protocols/Induc studies. (Created page with "{{Template:Team:Washington/Templates/Top}} =Induction Studies of Gene-Protein Fusions (mamK-sfGFP and mamI-sfGFP)= # Obtain a tube and fill it with 5 mL TB + Amp + Chlor. # Ob...")
- 22:16, 15 September 2011 (diff | hist) Team:Washington/Protocols (→Make It: Magnetosome Protocols)
- 22:04, 15 September 2011 (diff | hist) Team:Washington/Protocols/High PCR (→High-Yield PCR (Full-Gene Assembly)) (top)
- 18:13, 15 September 2011 (diff | hist) Team:Washington/Protocols/Overnights. (top)
- 03:37, 15 September 2011 (diff | hist) Team:Washington/Magnetosomes/Background
- 03:35, 15 September 2011 (diff | hist) Template:Team:Washington/Templates/Top
- 19:10, 14 September 2011 (diff | hist) Team:Washington/Protocols/Overnights.
- 19:10, 14 September 2011 (diff | hist) Team:Washington/Protocols/Overnights.
- 19:10, 14 September 2011 (diff | hist) N Team:Washington/Protocols/Micros. (Created page with "=Microscopy Slide Preparation= #") (top)
- 18:52, 14 September 2011 (diff | hist) N Team:Washington/Protocols/PBS. (Created page with "=Making PBS Stocks= # obtain and label a microcentrifuge tube. # Add 50 μL of Phosphate-Buffered Saline Broth (PBS). # From a plate, prick the desired colony and resuspend it ...")
- 18:31, 14 September 2011 (diff | hist) Team:Washington/Protocols/Elect. (top)
- 05:19, 14 September 2011 (diff | hist) Team:Washington/Protocols/Plas DNA. (top)
- 05:18, 14 September 2011 (diff | hist) Team:Washington/Protocols/High PCR
- 05:17, 14 September 2011 (diff | hist) Team:Washington/Protocols/Elect.
- 05:13, 14 September 2011 (diff | hist) Team:Washington/Protocols (→Make It: Magnetosome Protocols)
- 05:12, 14 September 2011 (diff | hist) N Team:Washington/Protocols/Elect. (Created page with "{{Template:Team:Washington/Templates/Top}} =Electroporation Protocol= # Obtain a competent cell aliquot from the -80oC freezer, keep the tubes on ice. # Add 40μL of ice cold...")
- 04:57, 14 September 2011 (diff | hist) Team:Washington/Protocols/Gib Rxn
- 04:57, 14 September 2011 (diff | hist) Team:Washington/Protocols/Gib Rxn
- 04:51, 14 September 2011 (diff | hist) Team:Washington/Protocols/Overnights. (→Preparation of Overnight Cultures)
- 04:51, 14 September 2011 (diff | hist) N Team:Washington/Protocols/Overnights. (Created page with "{{Template:Team:Washington/Templates/Top}} =Preparation of Overnight Cultures= #stuff")
- 04:47, 14 September 2011 (diff | hist) N Team:Washington/Protocols/High PCR (Created page with "{{Template:Team:Washington/Templates/Top}} =High-Yield PCR (Full-Gene Assembly)= # In an appropriately labeled tube, combine the following: ## 20 μL flash mastermix ## 2 μL...")
- 04:39, 14 September 2011 (diff | hist) Team:Washington/Protocols (→Make It: Magnetosome Protocols)
- 04:37, 14 September 2011 (diff | hist) N Team:Washington/Protocols/Plas DNA. (Created page with "{{Template:Team:Washington/Templates/Top}} =Isolation of Plasmid DNA (miniprep)= # Gently vortex overnight culture(s) to mix cells. # Pipet ~1 mL of cells into a labeled micro...")
- 04:19, 14 September 2011 (diff | hist) Team:Washington/Protocols/Gib Rxn
- 04:18, 14 September 2011 (diff | hist) Team:Washington/Protocols (→Make It: Magnetosome Protocols)
- 04:12, 14 September 2011 (diff | hist) Team:Washington/Protocols (→Make It: Magnetosome Protocols)
- 04:11, 14 September 2011 (diff | hist) Team:Washington/Protocols (→Make It: Magnetosome Protocols)
- 04:09, 14 September 2011 (diff | hist) Team:Washington/Protocols (→Make It: Magnetosome Protocols)
- 04:08, 14 September 2011 (diff | hist) Team:Washington/Protocols (→Make It: Magnetosome Protocols)
- 04:07, 14 September 2011 (diff | hist) Team:Washington/Protocols (→Make It: Magnetosome Protocols)
- 04:02, 14 September 2011 (diff | hist) Team:Washington/Protocols (→Make It: Magnetosome Protocols)
- 04:00, 14 September 2011 (diff | hist) Team:Washington/Protocols
- 03:59, 14 September 2011 (diff | hist) N Team:Washington/Protocols/Gib Rxn (Created page with "{{Template:Team:Washington/Templates/Top}} =Gibson Cloning/Assembly= # Add 15uL of Gibson MasterMix to each tube. # Add ~20-30 ng of purified Backbone DNA to the reaction tube...")
- 00:30, 13 September 2011 (diff | hist) Team:Washington/Magnetosomes/Background
- 22:11, 12 September 2011 (diff | hist) Team:Washington/Magnetosomes/Background
- 21:58, 12 September 2011 (diff | hist) Team:Washington/Protocols (→Make It: Magnetosome Protocols)
- 21:57, 12 September 2011 (diff | hist) Team:Washington/Protocols (→General Protocols)
- 21:55, 12 September 2011 (diff | hist) Team:Washington/Protocols (→Make It: Magnetosome Protocols)
- 21:54, 12 September 2011 (diff | hist) Team:Washington/Protocols
- 21:48, 12 September 2011 (diff | hist) Team:Washington/Protocols (→Magnets Protocols)
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