"
Team:UC Davis/Notebook/Week 11
From 2011.igem.org
Our Sponsors
Start a Family
Got a favorite BioBrick? Check our our process for expanding basic parts into part families.Criteria
View our judging criteria for iGEM 2011 here.
--Monday 8/22/11--
The digestion from last Friday looked good on a gel. After gel purifying it, we were finally able to ligate in the repressor mutants.
The digestion from last Friday looked good on a gel. After gel purifying it, we were finally able to ligate in the repressor mutants.
Today we also made more LB+Carb plates.
--Tuesday 8/23/11--
The next goal in construction is to take our 9 screening parts and putting them in a new backbone. Our construct is designed to end with a repressor, where it should end with a terminator. It is currently in pSB1A2 which does not include a terminator. The plan is to cut all of our parts at EcoR1 and Pst1 and ligate them into the pSB1C3 (also cut at EcoR1 and Pst1). Once this is done, we can go even further with our characterization.
The next goal in construction is to take our 9 screening parts and putting them in a new backbone. Our construct is designed to end with a repressor, where it should end with a terminator. It is currently in pSB1A2 which does not include a terminator. The plan is to cut all of our parts at EcoR1 and Pst1 and ligate them into the pSB1C3 (also cut at EcoR1 and Pst1). Once this is done, we can go even further with our characterization.
-Wednesday 8/24/11--
Last night, we stayed late after our 7 hour digestion to run the parts of a gel and purify them. However, it was 1:00 AM at this time so we decided to save the ligation and transformation for this morning. We transformed and plated all of our screening/characterizing constructs in pSB1C3 and will find out tomorrow if it was successful!
Last night, we stayed late after our 7 hour digestion to run the parts of a gel and purify them. However, it was 1:00 AM at this time so we decided to save the ligation and transformation for this morning. We transformed and plated all of our screening/characterizing constructs in pSB1C3 and will find out tomorrow if it was successful!
