Team:Paris Liliane Bettencourt/Notebook/2011/07/13/

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Kevin

Glycerol from lacO/tetO plasmids :

Centrifugation 10min - 4000 RPM
Put liquid on trash and vortex it
Resuspend with LB + Glycerol 20% (3mL on each tube)
Put it on cryovial tube (1,5mL on each tube -20°C / -80°C)

Cultur of glycerols :
10mL LB + 10μL Antibiotics
(List on 2011/07/12)

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