Team:NTNU Trondheim/LacI+pLac+mCherry

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Stress sensor precursor (LacI+pLac+mCherry)

This is our system without the rrnB P1-promotor. Because this construct lacks a downregulated promotor in front of it, LacI will not be syntethized, and in response, pLac will not be downregulated of LacI. Since pLac is not downregulated, mCherry is going to be present whether the cells are stressed or not. Our experiments indicate that the stress sensor precursor is put together the right way. The stress sensor precursor is made from the following biobricks:

Biobrick	Part number
LacI with RBS	BBa_J24679
Terminator	BBa_B0015
pLac	BBa_R0011
mCherry with RBS and terminator	BBa_J06702

The composite biobrick has a total length of 2153 bp, and is located in the plasmid pSB1A2.

This construct could in theory be used for any kind of biosensor. The only thing needed is a promotor that gets downregulated by the chemical one would want to detect.