Team:NTNU Trondheim/Stress Sensor

Revision as of 20:11, 22 August 2011 by Gunvor (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

• Home

• Team

  • About Us
  • Contact Us
  • Official Team Profile
  • Press

• Project

  • The Project
  • Modeling
  • Lab Journal
  • Lab Protocols
  • Modeling Journal

• Data

  • Data Page

  • Our BioBricks

    • Stress Sensor
    • LacI+pLac +mCherry
    • rrnB P1
    • relA
  • BioBricks Used
  • Defective Biobricks
  • Modeling Results

• Safety

• Attributions

• Sponsors

Stress sensor (rrnB+LacI+pLac+mCherry)

The stress sensor is our main construct. It is made from the following biobricks:

Biobrick	Part number
LacI with RBS	BBa_J24679
Terminator	BBa_B0015
pLac	BBa_R0011
mCherry with RBS and terminator	BBa_J06702

The promotor is a variant of BBa_K112118, that we have made ourselves using PCR. The stress sensor has a total length of 2653 bp, and is located inside the plasmid pSB1A2, carrying ampicillin resistance. We have put the stress sensor through several tests (see figure)

We have discovered that growing the cells containing the biosensor in a medium low in amino acids is the main way to activate it. As the figure shows, growing the cells in temperatures higher than 37 °C has little effect compared to growing them in a minimal medium. Already as we started this project we knew that lack of amino acids was the main trigger of ppGpp. Therefore, our results seems to indicate that our stress sensor work the way it is supposed to.