Team:Osaka/week3

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Revision as of 13:19, 17 August 2011 by Toshi (Talk | contribs)

August 16(Tue)

  1. Gel electrophoresis of 01,02,03,04,05,06
  2. Transfer to LB culture medium:01,02,03,04,05,06,1-23L
  3. Ligation
    • 8/5 3. the same way
  4. Transformation of Ligation products


August 17(Wed)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Restriction digests of 03,05
  5. Gel electrophoresis of digests
  6. Ligation
    • 07(C):05(upstream)+2-16N(downstream)+1-3A(vector)
  7. Transfer to LB culture midium:01,02,03,04,05,06
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