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From 2011.igem.org

Tuesday

Labwork

• Checked the TLC from yesterday, the result was not good. There was no oxime bands where we've added either tyrosine or phenylalanine as substrate to A1 and A2.

• User assembly: Checked the plates from yesterday, there were no colonies. We've contacted DTU to get some more DNA, enabling us to try again.

• Transfer overnight culture to TB media, growth until OD600 = 0,5. Added IPTG and 5-aminolevulinic acid hydrochloride, and moved the bottle to 28 degrees incubator. Took samples 1, 2 and 4 hours after the addition of IPTG and prepared samples for SDS-PAGE tomorrow. See protocol Expression & Purification in BL21

Other work

• Worked on the poster

• Worked on the illustrations

• Searched for hotel in Amsterdam

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