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From 2011.igem.org

Materials and Equipment for iGEM.

Here is a list that helps you prepare for the iGEM competition. This list has the materials needed and the equipment that will be used in the iGEM competition. Some of the reagents are generic because the project will specify what type of reagent you will use. Also the list is divided if you have a thermocycler or not.

Lab has a Thermocycler

Yeast

• Materials: this list is a beginning point for the lab. There are protocols for Ecoli and Yeast on openwetware that are helpful.
  • Growth media: YPD
  • Selective media: SD with amino acids added
  • Agar: YPD, Electrophoresis agar
  • Restriction enzymes: Biobrick kit from New England Science will help
  • Buffers: TAE/TBE/or LB
  • Seed DNA
  • Stains for gels
  • Enzymes: DNA and RNA Polymerase (if needed), ligase, protease
  • dNTP’s
  • Chemicals: MgCl, Tris-HCl
  • Compenent cells both ecoli and yeast

Equipment for the lab PCR, pressure cooker is being used as an autoclave, Micropipettes and tips, hemocytometer or spectrophotometer, Tubes, plates, Incubator, water bath or shaking water bath.

Ecoli

• Materials: this list is a beginning point for the lab. There are protocols for Ecoli and Yeast on openwetware that are helpful.
  • Growth Media: T broth, M9, LB
  • Selective Media: Antibiotics added to media
  • Agar: LB agar, Electrophoresis agar
  • Restriction enzymes: Biobrick kit from New England Science will help
  • Buffers: TAE/TBE/or LB
  • Stains for gels
  • Enzymes: DNA and RNA Polymerase (if needed), ligase, protease
  • dNTP’s
  • Chemicals: MgCl, Tris-HCl

Equipment for the lab PCR, pressure cooker is being used as an autoclave, Micropipettes and tips, hemocytometer or spectrophotometer, Tubes, plates, Incubator, water bath or shaking water bath.

No Thermocycler

• Waterbath
• Enzymes for 3A assembly method
• Growth reagents
• Incubator
• Selective Media
• Competent cells