Team:Osaka/week1
From 2011.igem.org
Contents |
August 1 (Mon)
- Preparation of LB agar plates (33 Amp, 18 Kan, 17 Cam).
August 2 (Tue)
- Culture medium preparation
- Competent cells preparation - Nojima Method
- SOB medium (MgCl2 not yet added) -> stored at 4˚C
- Preparation of glucose solution for making SOC medium.
- Single-colony streaking of E. coli (strain DH5α) on 2 LB agar plates -> 37˚C incubation o/n
Tomorrow we shall prepare the remainder of the reagents and inoculate SOB with o/n-incubated E. coli.
- Transformation of Registry parts (See Table 1).
Note: 'ID' is an internal identifier used by wet work members to simplify labeling etc. In the case of iGEM distribution parts it usually refers to the plate location.
ID | Part Name | Resistance | Description |
---|---|---|---|
1-9N | <bbpart>BBa_K118023</bbpart> | A | RecA promoter |
1-2M | <bbpart>BBa_K118022</bbpart> | A | RBS |
1-14K | <bbpart>BBa_B0034</bbpart> | A | GFP(wild type) |
1-12M | <bbpart>BBa_B0010</bbpart> | A | RBS+GFP+double terminator |
1-1D | <bbpart>BBa_R0010</bbpart> | A | promoter(lacI regulation) |
3-6H | <bbpart>BBa_E1010</bbpart> | A | CrtE I B with RBS |
2-18H | <bbpart>BBa_E1010</bbpart> | A | RBS+CrtE |
2-16N | <bbpart>BBa_E1010</bbpart> | A | RBS+CrtB |
2-18H | <bbpart>BBa_E1010</bbpart> | A | RBS+CrtI |
1-17P | <bbpart>BBa_E1010</bbpart> | A | RBS+CrtY |
1-1G | <bbpart>BBa_E1010</bbpart> | A | construction plasmid containing mRFP coding device |
1-3A | <bbpart>BBa_E1010</bbpart> | C | construction plasmid containing mRFP coding device |
1-5A | <bbpart>BBa_E1010</bbpart> | K | construction plasmid containing mRFP coding device |
August 3 (Wed)
- Competent cells preparation (continued)
- Inoculation of pre-culture SOB from o/n-incubated agar plate colonies.
- Transfer from pre-culture to growth culture.
August 4 (Thu)
- OD measurements throughout the day till required OD (0.3~0.7) was obtained.
- Completion of competent cells according to protocol.
August 5 (Fri)
August 6 (Sat)
- Transformation of Registry parts (See Table 1).
Note: 'ID' is an internal identifier used by wet work members to simplify labeling etc. In the case of iGEM distribution parts it usually refers to the plate location.
ID | Part Name | Resistance | Description |
---|---|---|---|
2-20J | <bbpart>BBa_K118023</bbpart> | A | C. fermi endocellulase Cen A coding |
2-20H | <bbpart>BBa_K118022</bbpart> | A | C. fermi exocellulase Cex coding |
1-2M | <bbpart>BBa_B0034</bbpart> | A | RBS |
1-13D | <bbpart>BBa_B0010</bbpart> | A | terminator |
1-1D | <bbpart>BBa_R0010</bbpart> | A | promoter |
1-18F | <bbpart>BBa_E1010</bbpart> | K | RFP coding |