User:Allancrossman/Gibson

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Revision as of 10:36, 5 August 2011 by Allancrossman (Talk | contribs)
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In the event that a Gibson assembly becomes desirable...

Plac-RBS-LacZ-RBS-INP[spacer]-malS

  • Templates:
    • Vector = pSB1C3
    • Plac-RBS-LacZ-RBS = <partinfo>BBa_K523005</partinfo>
    • INP[spacer] - make by cutting <partinfo>BBa_K523008</partinfo> with SpeI and annealing spacer3fs and spacer3rt
    • malS = <partinfo>BBa_K523001</partinfo>

Primers

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