Team:Paris Liliane Bettencourt/Notebook/2011/07/18/

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Edward

Minipreps

on BBa_I712074 (pT7), concentration of DNA=249.5ng/µL
on BBa_K145001 (T7 RNA polymerase), concentration of DNA=220.4ng/µL
on BBa_K143021 (RBS-spoVg colony 17), concentration of DNA=165.9ng/µL

Restriction Digest of RBS-spoVg and T7 RNA polymerase
- BBa_K145001 cut by XbaI and PstI
- BBa_K143079 cut by SpeI and PstI
  Final volume : 20 µL

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