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From 2011.igem.org

Thursday

Lab Work

• Run PCR on 1.4 (pink) to check the B1 primers, because they haven't worked yet. We used to much DNA, so we have to do one more time on monday.

• Double digest of 2.23.3 and 2.23.4 (purple)follwed by gelelectroforesis, byt it seems like, they digest havn't worked, so this step has to be done again.

• Transformation of A1,A2/Exp. vector into BL21 cells and A2,B1/pSB1C3 into XLblue cells.

Other Work

• Studied on the requirements for the characterization of the BioBricks.

• Meeting with Michael Lyngkjær about inducible E.coli promoters.

• Studied on USER cloning and primer design.

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