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From 2011.igem.org

July 31 2011

Track: Alpha-pinene synthase

Joe is troubleshooting his PCR reaction in preparation of his yeast and biobrick plasmid construction. This time he used an annealing temperature of 60 degree Celsius, the low range for Phusion Hot Start Polymerase. The results after gel verification show a supercoiled DNA above the 1kb ladder and a small non-specific high molecular weight smear near 6-8kb. This was unexpected as the pinene synathase should be around 2.1kb long. It is unknown why this may be. Next time, he will try increasing the extension time and a higher annealing temperature.

August 01 2011

Track: Alpha-pinene synthase

Yup... troubleshooting the previous PCR reaction again. Joe used an annealing temperature of 63 degree Celsius or 65 degree Celsius and