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From 2011.igem.org

Adrien Lhomme-Duchadeuil

First, an estimation of how much antibiotics is necessary for Bacillus subtilis. Appropriate concentration in antibiotics

• Chloramphemicol : 5-10 µg/mL
• Spec : 50-100 µg/mL
• Tetracyclin: 25 µg/mL
• Kanamycin: 5µg/mL
• Evm(?): 1µg/mL + 25 µg/mL lincomycin (Verify spelling)

Results of electroporation: IT WORKED after three trials!

• Positive control worked
• Negative controls didn't show any colonies except when put in the presence of TetR (qty:12,5 µg/mL or 5µg/mL) which was insufficient => I will re-plate the improbable survivors on plates of different tetracyclin concentrations to test resistance.
• Cm resistant strain have survived in low quatities at both 30 and 5 µg/mL of antibiotics
• It seems that even cuvettes that had an electric arc were able to produce a couple colonies, which means that it doesn't all the bacteria.

• To come: protocol and table of results